By Xueji Zhang,Haifeng Dong,Yaping Tian
This booklet summarizes microRNA (miRNA) biology in numerous pathological procedures, emphasizing the numerous capability functions of miRNA in diagnostics and prognostics, in addition to novel drug objectives. the normal ideas used for miRNA detection together with regular PCR, Northern blotting, microarray and clone equipment are addressed. contemporary rising techniques in miRNA detection and quantification with stronger flexibility and flexibility, similar to novel molecular organic recommendations and locked nucleic acid (LNA) transformed probes, in addition to nanotechnology-based methods, also are incorporated. The booklet additionally highlights the newest advances in clinical-related miRNA detection tools in residing cells, circulating blood and tissue, akin to in situ hybridization (ISH) and molecular imaging thoughts, that are priceless to explain the biogenesis and organic functionality of miRNAs in vivo. ultimately, the respective benefits and downsides of assorted detection thoughts during this fast-moving box are mentioned, besides the demanding situations and promising new directions.
This e-book deals a priceless source for analytical chemists, biologists and physicians fascinated about miRNA research.
Dr. Xueji Zhang and Dr. Haifeng Dong are Professors on the tuition of Chemistry & organic Engineering, college of technological know-how & expertise Beijing (USTB), China.
Dr. Yaping Tian is a Professor on the division of medical Biochemistry, chinese language PLA common health center and armed forces scientific institution, China.